home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9407a.zip
/
M9470007.TXT
< prev
next >
Wrap
Text File
|
1994-07-02
|
3KB
|
44 lines
Document 0007
DOCN M9470007
TI Allergen-induced expression of cell surface markers on lymphocytes of
Chi t I-sensitized patients.
DT 9409
AU Liebers V; Raulf M; Baur X; Berufsgenossenschaftliches
Forschungsinstitut fur Arbeitsmedizin; (BGFA), Ruhr-Universitat Bochum,
Germany.
SO Allergy. 1994 Mar;49(3):163-9. Unique Identifier : AIDSLINE MED/94256625
AB Lymphocyte cultures of persons sensitized to the hemoglobin allergen Chi
t I show a highly significant response to the allergen measured in the
lymphocyte stimulation assay by (3H)-thymidine uptake. In this study, we
investigated by flow cytometry the expression of different cell surface
markers on lymphocytes after in vitro stimulation for 7 d with or
without the allergen Chi t I. We determined the expression of the
low-affinity receptor for IgE (CD23) on lymphocytes of Chi t
I-sensitized patients and Chi t I-exposed as well as nonexposed
controls. CD23 expression was significantly higher in patients than in
nonexposed controls. Exposed but healthy subjects showed intermediate
values. We also determined the expression of activation markers CD25
(IL-2 receptor) and HLA-DR on the lymphocytes of patients and nonexposed
controls. HLA-DR expression on non-T cells (CD3-) was significantly
higher in patients than in controls. HLA-DR on T cells (CD3+), and CD25
as well as CD23 expression, could be significantly enhanced after
antigen-specific stimulation in patients but not in controls, whereas
alpha/beta-T-cell-receptor expression was significantly reduced in
patients. Differences between patients and controls were not observed in
response to tetanus toxoid (TT) and phytohemagglutinin (PHA). Our
results demonstrate antigen-specific influences on the expression of
cell surface molecules. These findings may be valuable diagnostic
information.
DE Allergens/*IMMUNOLOGY Animal Antigens,
Surface/*BIOSYNTHESIS/IMMUNOLOGY B-Lymphocytes/*IMMUNOLOGY/METABOLISM
Cells, Cultured Chironomidae/*IMMUNOLOGY Comparative Study CD4-CD8
Ratio Flow Cytometry Hemoglobins/*IMMUNOLOGY Human HLA-DR
Antigens/BIOSYNTHESIS IgE/BIOSYNTHESIS Lymphocyte Transformation
Phytohemagglutinins/IMMUNOLOGY Receptors, Interleukin-2/*BIOSYNTHESIS
Support, Non-U.S. Gov't T-Lymphocytes/*IMMUNOLOGY/METABOLISM Tetanus
Toxoid/IMMUNOLOGY Time Factors JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).